PLN70-1KT GenElute™ Plasmid Miniprep Kit

PLN70-1KT GenElute™ Plasmid Miniprep Kit

sufficient for 70 purifications Features and Benefits : • Purified plasmid DNA in less than 30 minutes • Purify up to 15 μg of plasmid DNA • No detectable genomic DNA or RNA contamination • No phenol/chloroform extraction or alcohol precipitation required The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium. An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

Brand : Sigma

C.A.S. No.:

Packing : 1KIT