BK101-1KT ImmunoProbe™ Biotinylation Kit

BK101-1KT ImmunoProbe™ Biotinylation Kit

Kit includes all reagents necessary for biotinylation and for determination of biotin/protein molar ratio. For custom biotinylation of proteins, Sigma offers kits for conjugation on two different scales. The biotin-avidin system has become popular when high sensitivity and specificity are desired. Biotin can be conjugated to antibodies, lectins, enzymes, and other proteins. The protocols in the kits have been optimized for antibodies. Avidin binds to biotin with a high affinity (Ka = 1015 M) and specificity. When conjugated to enzymes or fluorochromes avidin provides a means of identifying biotinylated compounds via enzymatic conversion of substrate to form a visible product or detection of fluorescence by spectrophotometry or flow cytometry.

Brand : Sigma

C.A.S. No.:

Packing : 1KIT